The following is a list of nutrients involved, whether directly or indirectly, in methylation. Please consult a health care practitioner before making any changes to your diet or supplement regimen.

Vitamin B12 - Methylcobalamin

Vitamin B12, specifically in the form of methylcobalamin, plays a crucial role in various bodily functions. It is essential for the production of DNA and red blood cells, as well as the proper functioning of the nervous system. In terms of methylation, cobalamin is involved in the conversion of homocysteine to methionine, which is an important step in the methylation cycle.

Cyanocobalamin is the synthetic form of Vitamin B12. It takes more energy from your body to convert it into a form that your body can use. Being two steps away from "usable" by your body, it's best to avoid cyanocobalamin. It is most often found in sports and energy drinks, as a common supplement (the front label will say B12, so check the back label) or part of a multivitamin, and some fortified foods.

Vitamin B9 - Methylfolate / 5-MTHF

Vitamin B9, also known as methylfolate (as opposed to folic acid, which is the synthetic form), is necessary for DNA synthesis and methylation. Methylfolate is the active form of folate and is directly involved in methylation reactions. It acts as a methyl donor, contributing methyl groups to support the methylation process and regulate gene expression.

See the difference between the different forms

Vitamin B6 - Pyridoxine

Vitamin B6, or pyridoxine, is involved in a wide range of biochemical reactions in the body. It is necessary for amino acid metabolism, neurotransmitter synthesis (dopamine, serotonin, norepinephrine, GABA), and the production of hemoglobin. While B6 itself is not directly involved in the methylation process, it plays a supportive role by assisting in the conversion of homocysteine to cysteine. Deficiencies in B6 can lead to less-effective methylation (mood) balancing, skin issues, anemia, and impaired immune function.

While it is best to get nutrients within your diet, sometimes it's necessary to supplement what you can't get through diet. The most effective form of B6 is P5P. Other forms may be effective, but please consult with a certified health care practitioner before making changes to your supplements.

Vitamin B3 - Niacin

Vitamin B3, also known as niacin, is essential for energy production and the maintenance of healthy skin, nerves, and digestive system. Niacin supports methylation indirectly by helping to convert dietary tryptophan into niacinamide, which is a precursor to NAD+ (nicotinamide adenine dinucleotide), a coenzyme involved in methylation reactions. It supports energy production, DNA repair, and the regulation of gene expression. Niacin is indirectly linked to methylation through its involvement in the NAD+ coenzyme, which plays a role in methylation reactions.

Additionally, niacin plays a crucial role in supporting cardiovascular health. It can help lower LDL (low-density lipoprotein) cholesterol, often referred to as "bad" cholesterol, while increasing HDL (high-density lipoprotein) cholesterol, known as "good" cholesterol. This can contribute to a healthier lipid profile and reduce the risk of cardiovascular diseases. Niacin also supports the proper functioning of the nervous system by participating in the synthesis of neurotransmitters, including serotonin, which plays a role in mood regulation. Moreover, niacin is involved in DNA repair, cell signaling, and the maintenance of healthy skin. Niacin can be obtained from various food sources, including meat, poultry, fish, nuts, seeds, and whole grains. In some cases, supplementation may be necessary, especially for individuals with specific dietary needs or medical conditions. However, it's important to consult with a healthcare professional before starting niacin supplementation, as high doses can have side effects and may interact with certain medications.

Vitamin B2 - Riboflavin

Vitamin B2, or riboflavin, is necessary for energy production, cellular growth, and the metabolism of fats, carbohydrates, and proteins. While it is not directly involved in methylation, riboflavin is a component of the flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) coenzymes, which participate in various enzymatic reactions, including those related to methylation.

One of the key advantages of vitamin B2 is its involvement in energy production. It serves as a coenzyme in various metabolic reactions, helping convert carbohydrates, fats, and proteins into usable energy for the body. Additionally, vitamin B2 acts as an antioxidant, helping to protect cells from damage caused by harmful molecules called free radicals. This antioxidant activity contributes to maintaining healthy skin, eyes, and other tissues. Vitamin B2 also supports the health of the nervous system, playing a role in the production and regulation of certain neurotransmitters. B2 is essential for the normal growth and development of the body, including the formation of red blood cells. It also supports the health of the mucous membranes, aiding in the maintenance of a healthy digestive system. Vitamin B2 can be obtained through a balanced diet, including foods like dairy products, lean meats, green leafy vegetables, and whole grains. In some cases, supplementation may be necessary, especially for individuals with certain dietary restrictions or increased nutrient needs. As always, it's advisable to consult with a healthcare professional to determine the appropriate dosage and usage of vitamin B2 supplements.

Vitamin C

Vitamin C, also known as ascorbic acid, is an important antioxidant that supports immune function, collagen synthesis, and iron absorption. Although it is not directly involved in methylation, vitamin C helps to regenerate and maintain the activity of other essential molecules, such as folate and vitamin E, which indirectly support the methylation process. Taking Vitamin C as a supplement will lower your methylation.

Betaine (TMG)

Betaine, also known as trimethylglycine (TMG), is a naturally occurring compound found in various foods. It functions as a methyl donor and can support methylation reactions by contributing methyl groups. Betaine is also involved in maintaining healthy liver function and cellular osmolyte balance. Taking betaine as a supplement will raise your methylation.

Some foods containing betaine include:

  1. Beets: Beets are a great source of TMG. You can enjoy them in salads, roasted, or blended into smoothies.
  2. Spinach: Leafy greens like spinach are not only rich in essential nutrients but also contain TMG. Include spinach in salads, sautés, or add it to your favorite smoothie.
  3. Quinoa: This nutritious grain is not only a good source of protein and fiber but also contains TMG. Use quinoa as a base for salads, as a side dish, or in casseroles.
  4. Wheat bran: Wheat bran, the outer layer of wheat grains, is high in TMG. It can be added to cereals, baked goods, or used as a fiber-rich topping for yogurt or smoothies.
  5. Seafood: Fish and seafood, such as salmon, shrimp, and cod, contain TMG. Including these in your diet can provide not only TMG but also omega-3 fatty acids and other important nutrients.
  6. Whole grains: Whole grains like oats, barley, and brown rice also contain TMG. Incorporate these grains into your meals as a side dish, in soups, or as a breakfast option.
  7. Amaranth: Amaranth is a gluten-free grain that is high in TMG. It can be cooked and used as a base for salads, porridge, or added to baked goods.
  8. Lentils: Lentils are a versatile legume that provides TMG along with protein and fiber. They can be used in soups, stews, salads, or made into flavorful spreads like hummus.
  9. Brussels sprouts: Brussels sprouts are a cruciferous vegetable that contains TMG. Roasting or sautéing them can bring out their delicious flavors.
  10. Sweet potatoes: Sweet potatoes are not only tasty but also a good source of TMG. Enjoy them baked, roasted, or mashed as a nutritious side dish.


SAMe (S-adenosylmethionine) is a molecule that serves as a methyl donor in numerous biochemical reactions, including methylation. It plays a critical role in DNA methylation, neurotransmitter synthesis, and the regulation of gene expression. SAMe is involved in the methylation of DNA, RNA, proteins, and other molecules, contributing to various physiological processes. Taking SAMe as a supplement will raise your methylation.


NAC (N-acetyl cysteine) is a precursor to glutathione, a powerful antioxidant that supports detoxification processes in the body. While it is not directly involved in methylation, NAC indirectly supports methylation by promoting the availability of cysteine, an amino acid used in the synthesis of glutathione, which is essential for methylation reactions. For those with  transsulfurationpathways (detox) issues, NAC will raise methylation for a few hours.


L-theanine is an amino acid primarily found in tea leaves, but also as a supplement. It has a calming effect on the nervous system and is known to promote relaxation and reduce stress. While L-theanine itself is not directly involved in methylation, its ability to induce a state of relaxation may indirectly support the methylation process. Chronic stress can disrupt methylation patterns, so managing stress levels with compounds like L-theanine may help maintain optimal methylation.

One of the main advantages of taking L-theanine as a supplement is its ability to induce a sense of calmness without causing drowsiness. It can help promote a relaxed state by increasing the production of neurotransmitters like serotonin and dopamine, which are associated with mood regulation. This can be particularly beneficial for managing anxiety and stress. L-theanine also has the potential to enhance focus and attention, as it can cross the blood-brain barrier and affect brain wave patterns. It may help improve cognitive performance without the jittery side effects associated with other stimulants. Additionally, L-theanine has been suggested to support sleep quality by promoting relaxation and reducing feelings of restlessness. While further research is needed to fully understand the effects of L-theanine, it appears to be a promising supplement for individuals seeking relaxation, stress reduction, and improved focus without sedation. As always, consult with a healthcare professional before starting any new supplements.


Magnesium is an essential mineral that is involved in over 300 biochemical reactions in the body. It plays a vital role in energy production, nerve function, muscle contraction, and the maintenance of bone health. While magnesium is not directly involved in methylation, it is important for overall cellular function, including processes that indirectly support methylation. Getting enough magnesium in the diet can be difficult, so talk with a health care practitioner about adding supplemental magnesium to your regimen before making any changes to your diet/supplement regimen.

Ceylon Cinnamon

Ceylon Cinnamon is a type of cinnamon known for its distinct flavor and potential health benefits. It contains various compounds, including cinnamaldehyde, that have antioxidant and anti-inflammatory properties. While cinnamon itself is not directly involved in methylation, its antioxidant properties help protect cells from oxidative stress as well as protecting against inflammation, which can negatively affect methylation patterns.

When consuming Ceylon cinnamon, it is important to consider the source and quality of the product. Look for reputable brands that specifically label it as Ceylon or Cinnamomum verum to ensure you are getting the genuine product. As with any dietary supplement or addition to your diet, it is advisable to consult with a healthcare professional, particularly if you have any underlying health conditions or are taking medications.

Chromium (Picolinate)

Chromium is a trace mineral that plays a role in carbohydrate and lipid metabolism. While chromium is not directly involved in methylation, it contributes to overall metabolic processes that indirectly influence methylation.

In addition to methylation, one of the key benefits of chromium supplementation is its role in blood sugar regulation. Chromium enhances the action of insulin, a hormone responsible for transporting glucose into cells for energy production. By improving insulin sensitivity, chromium supplementation may help stabilize blood sugar levels, which is particularly beneficial for individuals with diabetes or insulin resistance. Furthermore, chromium has been associated with promoting healthy weight management by potentially reducing food cravings and supporting metabolism. It may also aid in maintaining lean muscle mass during weight loss efforts. Additionally, chromium has been linked to improved lipid profile, as it may help lower LDL cholesterol and increase HDL cholesterol levels. While more research is needed to fully understand the mechanisms and effects of chromium supplementation, it shows promise in various aspects of metabolic health, making it a popular choice among individuals seeking to support their overall well-being. As always, it's recommended to consult with a healthcare professional before starting any new supplements.


Calcium is a mineral essential for healthy bones and teeth, as well as proper muscle and nerve function. While calcium is not directly involved in methylation, it is involved in cellular signaling pathways and enzyme activation that can indirectly affect methylation processes.


Indole-3-Carbinol (I3C) is a compound found in cruciferous vegetables like broccoli, cauliflower, and cabbage. It has been studied for its potential anticancer properties. While I3C is not directly involved in methylation, it can affect gene expression and metabolism, which can indirectly influence methylation patterns.

In addition to the foods that contain I3C, it can also be taken as a supplement. One important benefit of taking I3C as a supplement is that it may help regulate estrogen levels in the body. It can change a strong form of estrogen called estradiol into weaker forms, which could help keep hormones in balance. This is particularly useful for people with too much estrogen or those wanting to support breast health. I3C also has antioxidant and anti-inflammatory properties, which means it might protect cells from damage and reduce inflammation in the body. It could also help the liver remove toxins from the body. Although more research is needed, these early findings suggest that taking I3C as a supplement could be helpful for maintaining healthy hormone levels and overall cell health. Remember to talk to a healthcare professional before starting any new supplements or making changes to your diet.

Sodium Bicarbonate

Acidosis is a medical condition characterized by an excessive amount of acid in the body fluids, leading to a decrease in the body's pH. Symptoms can include confusion, fatigue, shortness of breath, muscle weakness. Persistent acidosis can affect various body functions and systems, including cardiovascular and respiratory systems, and can interfere with enzyme activity and metabolism. Sodium bicarbonate can help to ease the symptoms of acidosis, but doesn't treat the underlying issue.

Protein + Sugar

Consuming protein and sugar together can, for some people, be an issue that leads to digestive and cognitive issues. Symptoms include nausea, vomiting, diarrhea, cramping, foggy-headedness, among others. Common pairings of protein + sugar are protein shakes, meat with sweet sauces (like orange chicken, glazed ham), BBQ ribs, eggs and ketchup (when it contains sugar), and protein bars.

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